4A7E

X-ray crystal structure of porcine insulin flash-cooled at high pressure

Summary for 4A7E

• Entry DOI: 10.2210/pdb4a7e/pdb
• Related: 1B17 1B18 1B19 1B2A 1B2B 1B2C 1B2D 1B2E 1B2F 1B2G 1DEI 1IZA 1IZB 1M5A 1MPJ 1SDB 1WAV 1ZEI 1ZNI 2TCI 3INS 3MTH 4A7D 4INS 7INS 9INS
• Descriptor: INSULIN A CHAIN, INSULIN B CHAIN (3 entities in total)
• Functional Keywords: hormone, high-pressure cooling, high-pressure protein crystallography
• Biological source: SUS SCROFA (PIG); More
• Cellular location: Secreted: P01315 P01315
• Total number of polymer chains: 2
• Total formula weight: 5787.63

Authors

Burkhardt, A.,Warmer, M.,Panneerselvam, S.,Wagner, A.,Reimer, R.,Hohenberg, H.,Meents, A. (deposition date: 2011-11-14, release date: 2011-11-30, Last modification date: 2024-10-09)

Primary citation

Burkhardt, A.,Warmer, M.,Pannerselvam, S.,Wagner, A.,Zouni, A.,Reimer, R.,Hohenberg, H.,Meents, A.
Fast High-Pressure Freezing of Protein Crystals in Their Mother Liquor
Acta Crystallogr.,Sect.F, 68:495-, 2012

PubMed Abstract: High-pressure freezing (HPF) is a method which allows sample vitrification without cryoprotectants. In the present work, protein crystals were cooled to cryogenic temperatures at a pressure of 210 MPa. In contrast to other HPF methods published to date in the field of cryocrystallography, this protocol involves rapid sample cooling using a standard HPF device. The fast cooling rates allow HPF of protein crystals directly in their mother liquor without the need for cryoprotectants or external reagents. HPF was first attempted with hen egg-white lysozyme and cubic insulin crystals, yielding good to excellent diffraction quality. Non-cryoprotected crystals of the membrane protein photosystem II have been successfully cryocooled for the first time. This indicates that the presented HPF method is well suited to the vitrification of challenging systems with large unit cells and weak crystal contacts.

PubMed: 22505429
DOI: 10.1107/S1744309112009670

Experimental method

X-RAY DIFFRACTION (1.856 Å)