437D

CRYSTAL STRUCTURE OF AN RNA PSEUDOKNOT FROM BEET WESTERN YELLOW VIRUS INVOLVED IN RIBOSOMAL FRAMESHIFTING

Summary for 437D

• Entry DOI: 10.2210/pdb437d/pdb
• Descriptor: RNA PSEUDOKNOT, MAGNESIUM ION, SODIUM ION, ... (4 entities in total)
• Functional Keywords: pseudoknot, rna, triplex, viral frameshifting
• Total number of polymer chains: 1
• Total formula weight: 9292.78

Authors

Su, L.,Chen, L.,Egli, M.,Berger, J.M.,Rich, A. (deposition date: 1998-11-24, release date: 1998-12-03, Last modification date: 2024-02-28)

Primary citation

Su, L.,Chen, L.,Egli, M.,Berger, J.M.,Rich, A.
Minor groove RNA triplex in the crystal structure of a ribosomal frameshifting viral pseudoknot.
Nat.Struct.Biol., 6:285-292, 1999

PubMed Abstract: Many viruses regulate translation of polycistronic mRNA using a -1 ribosomal frameshift induced by an RNA pseudoknot. A pseudoknot has two stems that form a quasi-continuous helix and two connecting loops. A 1.6 A crystal structure of the beet western yellow virus (BWYV) pseudoknot reveals rotation and a bend at the junction of the two stems. A loop base is inserted in the major groove of one stem with quadruple-base interactions. The second loop forms a new minor-groove triplex motif with the other stem, involving 2'-OH and triple-base interactions, as well as sodium ion coordination. Overall, the number of hydrogen bonds stabilizing the tertiary interactions exceeds the number involved in Watson-Crick base pairs. This structure will aid mechanistic analyses of ribosomal frameshifting.

PubMed: 10074948
DOI: 10.1038/6722

Experimental method

X-RAY DIFFRACTION (1.6 Å)