437D
CRYSTAL STRUCTURE OF AN RNA PSEUDOKNOT FROM BEET WESTERN YELLOW VIRUS INVOLVED IN RIBOSOMAL FRAMESHIFTING
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-06-14 |
| Detector | FUJI |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 30.080, 30.080, 140.080 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 1.600 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.25400 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.870 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.070 | 0.165 |
| Number of reflections | 8793 | |
| Completeness [%] | 93.0 | 69.9 * |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 25 * | pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MGCL2 | ||
| 2 | 1 | 1 | 2-BUTANOL | ||
| 3 | 1 | 1 | POTASSIUM MOPS | ||
| 4 | 1 | 1 | SPERMIDINE | ||
| 5 | 1 | 2 | 2-BUTANOL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | RNA | 4 (mg/ml) | |
| 2 | 1 | drop | 5 (mM) | ||
| 3 | 1 | drop | sec-butanol | 5 (%) | |
| 4 | 1 | drop | potassium MOPS | 100 (mM) | |
| 5 | 1 | drop | spermidine | 2 (mM) | |
| 6 | 1 | reservoir | sec-butanol | 18 (%) |
