421D

5'-D(*TP*TP*CP*TP*TP*(BRO)CP*TP*TP*C)-3', 5'-R(*GP*AP*AP*GP*AP*AP*GP*AP*A)-3'

Summary for 421D

• Entry DOI: 10.2210/pdb421d/pdb
• Descriptor: DNA (5'-D(*TP*TP*CP*TP*TP*(CBR)P*TP*TP*C)-3'), RNA (5'-R(*GP*AP*AP*GP*AP*AP*GP*AP*A)-3') (3 entities in total)
• Functional Keywords: dna:rna hybrid duplex, dna-rna complex, dna-rna hybrid
• Total number of polymer chains: 2
• Total formula weight: 5692.54

Authors

Xiong, Y.,Sundaralingam, M. (deposition date: 1998-08-16, release date: 1999-01-05, Last modification date: 2023-12-27)

Primary citation

Xiong, Y.,Sundaralingam, M.
Crystal structure and conformation of a DNA-RNA hybrid duplex with a polypurine RNA strand: d(TTCTTBr5CTTC)-r(GAAGAAGAA).
Structure, 6:1493-1501, 1998

PubMed Abstract: . DNA-RNA hybrids are substrates for RNase H. This enzyme catalyzes the hydrolysis of the RNA strand in the hybrid form. The polypurine tract (PPT) in human immunodeficiency virus 1 (HIV-1) is a short stretch of purines ( approximately 15 bases) located at the 3'-end of the U3 region of the RNA genome. The PPT has the unique ability to resist digestion by RNase H and serves as a primer for plus-strand DNA synthesis.

PubMed: 9862803
DOI: 10.1016/S0969-2126(98)00148-8

Experimental method

X-RAY DIFFRACTION (1.8 Å)