421D
5'-D(*TP*TP*CP*TP*TP*(BRO)CP*TP*TP*C)-3', 5'-R(*GP*AP*AP*GP*AP*AP*GP*AP*A)-3'
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 263 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-02-15 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 61 |
| Unit cell lengths | 48.080, 48.080, 42.880 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.23700 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 7.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.040 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.069 | 0.355 |
| Number of reflections | 4948 | |
| Completeness [%] | 89.3 | 77.2 |
| Redundancy | 3.46 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | pH 7.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | hybrid | 1 (mM) | double strand |
| 2 | 1 | drop | cobalt hexamine | 0.5 (mM) | |
| 3 | 1 | drop | spermine tetrachloride | 5 (mM) | |
| 4 | 1 | drop | sodium cacodylate | 80 (mM) | pH7.0 |
| 5 | 1 | drop | MPD | 10 (%(v/v)) | |
| 6 | 1 | reservoir | MPD | 40 (%) |
