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3ZFQ

Crystal structure of product-like, processed N-terminal protease Npro with mercury

Summary for 3ZFQ
Entry DOI10.2210/pdb3zfq/pdb
Related3ZFN 3ZFO 3ZFP 3ZFR 3ZFT 3ZFU
DescriptorN-TERMINAL PROTEASE NPRO, MONOTHIOGLYCEROL, MERCURY (II) ION, ... (4 entities in total)
Functional Keywordshydrolase, auto-processing cysteine protease, viral protease, in cis- cleavage, hydroxide-dependent catalysis, auto-proteolysis, immune modulation, host-pathogen interaction, convergent evolution
Biological sourcePESTIVIRUS STRAIN D32/00_HOBI
Total number of polymer chains1
Total formula weight16866.78
Authors
Zogg, T.,Sponring, M.,Schindler, S.,Koll, M.,Schneider, R.,Brandstetter, H.,Auer, B. (deposition date: 2012-12-12, release date: 2013-05-15, Last modification date: 2024-11-13)
Primary citationZogg, T.,Sponring, M.,Schindler, S.,Koll, M.,Schneider, R.,Brandstetter, H.,Auer, B.
Crystal Structures of the Viral Protease Npro Imply Distinct Roles for the Catalytic Water in Catalysis
Structure, 21:929-, 2013
Cited by
PubMed Abstract: Npro is a key effector protein of pestiviruses such as bovine viral diarrhea virus and abolishes host cell antiviral defense mechanisms. Synthesized as the N-terminal part of the viral polyprotein, Npro releases itself via an autoproteolytic cleavage, triggering its immunological functions. However, the mechanisms of its proteolytic action and its immune escape were unclear. Here, we present the crystal structures of Npro to 1.25 Å resolution. Structures of pre- and postcleavage intermediates identify three catalytically relevant elements. The trapping of the putative catalytic water reveals its distinct roles as a base, acid, and nucleophile. The presentation of the substrate further explains the enigmatic latency of the protease, ensuring a single in cis cleavage. Additionally, we identified a zinc-free, disulfide-linked conformation of the TRASH motif, an interaction hub of immune factors. The structure opens additional opportunities in utilizing Npro as an autocleaving fusion protein and as a pharmaceutical target.
PubMed: 23643950
DOI: 10.1016/J.STR.2013.04.003
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.65 Å)
Structure validation

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