3ZEP

Crystal Structure of JAK3 Kinase Domain in Complex with a Pyrrolopyrazine-2-phenyl Ether Inhibitor

Summary for 3ZEP

• Entry DOI: 10.2210/pdb3zep/pdb
• Descriptor: TYROSINE-PROTEIN KINASE JAK3, 2-[[(3R)-3-acetamido-2,3-dihydro-1H-inden-5-yl]oxy]-N-[(1S)-1-cyclopropylethyl]-5H-pyrrolo[2,3-b]pyrazine-7-carboxamide, GLYCEROL, ... (4 entities in total)
• Functional Keywords: transferase, stat5, stat6, interleukin-2, common-gamma chain, atp site kinase inhibitor, cancer, scid, severe combined immunodeficiency
• Biological source: HOMO SAPIENS (HUMAN)
• Cellular location: Endomembrane system ; Peripheral membrane protein : P52333
• Total number of polymer chains: 4
• Total formula weight: 133664.06

Authors

Kuglstatter, A.,Jestel, A.,Nagel, S.,Boettcher, J.,Blaesse, M. (deposition date: 2012-12-06, release date: 2013-12-11, Last modification date: 2024-10-23)

Primary citation

Jaime-Figueroa, S.,De Vicente, J.,Hermann, J.,Jahangir, A.,Jin, S.,Kuglstatter, A.,Lynch, S.M.,Menke, J.,Niu, L.,Patel, V.,Shao, A.,Soth, M.,Vu, M.D.,Yee, C.
Discovery of a Series of Novel 5H-Pyrrolo[2,3-B]Pyrazine-2-Phenyl Ethers, as Potent Jak3 Kinase Inhibitors.
Bioorg.Med.Chem.Lett., 23:2522-, 2013

PubMed Abstract: We report the discovery of a novel series of ATP-competitive Janus kinase 3 (JAK3) inhibitors based on the 5H-pyrrolo[2,3-b]pyrazine scaffold. The initial leads in this series, compounds 1a and 1h, showed promising potencies, but a lack of selectivity against other isoforms in the JAK family. Computational and crystallographic analysis suggested that the phenyl ether moiety possessed a favorable vector to achieve selectivity. Exploration of this vector resulted in the identification of 12b and 12d, as potent JAK3 inhibitors, demonstrating improved JAK family and kinase selectivity.

PubMed: 23541670
DOI: 10.1016/J.BMCL.2013.03.015

Experimental method

X-RAY DIFFRACTION (2.35 Å)