3ZD7

Snapshot 3 of RIG-I scanning on RNA duplex

Summary for 3ZD7

• Entry DOI: 10.2210/pdb3zd7/pdb
• Related: 3ZD6
• Descriptor: PROBABLE ATP-DEPENDENT RNA HELICASE DDX58, RNA DUPLEX, ZINC ION, ... (6 entities in total)
• Functional Keywords: hydrolase-rna complex, helicase, innate immunity, hydrolase/rna
• Biological source: HOMO SAPIENS (HUMAN); More
• Cellular location: Cytoplasm: O95786
• Total number of polymer chains: 3
• Total formula weight: 86642.67

Authors

Luo, D.,Pyle, A.M. (deposition date: 2012-11-25, release date: 2013-08-07, Last modification date: 2024-11-20)

Primary citation

Kohlway, A.,Luo, D.,Rawling, D.C.,Ding, S.C.,Pyle, A.M.
Defining the Functional Determinants for RNA Surveillance by Rig-I.
Embo Rep., 14:772-, 2013

PubMed Abstract: Retinoic acid-inducible gene-I (RIG-I) is an intracellular RNA sensor that activates the innate immune machinery in response to infection by RNA viruses. Here, we report the crystal structure of distinct conformations of a RIG-I:dsRNA complex, which shows that HEL2i-mediated scanning allows RIG-I to sense the length of RNA targets. To understand the implications of HEL2i scanning for catalytic activity and signalling by RIG-I, we examined its ATPase activity when stimulated by duplex RNAs of varying lengths and 5' composition. We identified a minimal RNA duplex that binds one RIG-I molecule, stimulates robust ATPase activity, and elicits a RIG-I-mediated interferon response in cells. Our results reveal that the minimal functional unit of the RIG-I:RNA complex is a monomer that binds at the terminus of a duplex RNA substrate. This behaviour is markedly different from the RIG-I paralog melanoma differentiation-associated gene 5 (MDA5), which forms cooperative filaments.

PubMed: 23897087
DOI: 10.1038/EMBOR.2013.108

Experimental method

X-RAY DIFFRACTION (2.5 Å)