3ZBO

A new family of proteins related to the HEAT-like repeat DNA glycosylases with affinity for branched DNA structures

3ZBO の概要

• エントリーDOI: 10.2210/pdb3zbo/pdb
• 分子名称: ALKF, CHLORIDE ION (3 entities in total)
• 機能のキーワード: unknown function, dna damage, base excision repair, alkc, alkd, holliday junctions
• 由来する生物種: BACILLUS CEREUS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 54203.40

構造登録者

Backe, P.H.,Simm, R.,Laerdahl, J.K.,Dalhus, B.,Fagerlund, A.,Okstad, O.A.,Rognes, T.,Alseth, I.,Kolsto, A.-B.,Bjoras, M. (登録日: 2012-11-12, 公開日: 2013-05-15, 最終更新日: 2023-12-20)

主引用文献

Backe, P.H.,Simm, R.,Laerdahl, J.K.,Dalhus, B.,Fagerlund, A.,Okstad, O.A.,Rognes, T.,Alseth, I.,Kolsto, A.-B.,Bjoras, M.
A New Family of Proteins Related to the Heat-Like Repeat DNA Glycosylases with Affinity for Branched DNA Structures.
J.Struct.Biol., 183:66-, 2013

PubMed Abstract: The recently discovered HEAT-like repeat (HLR) DNA glycosylase superfamily is widely distributed in all domains of life. The present bioinformatics and phylogenetic analysis shows that HLR DNA glycosylase superfamily members in the genus Bacillus form three subfamilies: AlkC, AlkD and AlkF/AlkG. The crystal structure of AlkF shows structural similarity with the DNA glycosylases AlkC and AlkD, however neither AlkF nor AlkG display any DNA glycosylase activity. Instead, both proteins have affinity to branched DNA structures such as three-way and Holliday junctions. A unique β-hairpin in the AlkF/AlkG subfamily is most likely inserted into the DNA major groove, and could be a structural determinant regulating DNA substrate affinity. We conclude that AlkF and AlkG represent a new family of HLR proteins with affinity for branched DNA structures.

PubMed: 23623903
DOI: 10.1016/J.JSB.2013.04.007

実験手法

X-RAY DIFFRACTION (1.58 Å)