3X00
Crystal structure of the core streptavidin mutant V212 (Y22S/N23D/S27D/S45N/Y83S/R84K/E101D/R103K/E116N) complexed with bis iminobiotin long tail (Bis-IMNtail) at 1.3 A resolution
Summary for 3X00
Entry DOI | 10.2210/pdb3x00/pdb |
Related | 3WZN 3WZO 3WZP 3WZQ |
Descriptor | Streptavidin, 6-({5-[(2E,3aS,4S,6aR)-2-iminohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoyl}amino)hexanoic acid, ETHANE-1,2-DIAMINE, ... (4 entities in total) |
Functional Keywords | beta-barrel, biotin binding protein |
Biological source | Streptomyces avidinii |
Cellular location | Secreted: P22629 |
Total number of polymer chains | 4 |
Total formula weight | 62788.40 |
Authors | Kawato, T.,Mizohata, E.,Shimizu, Y.,Meshizuka, T.,Yamamoto, T.,Takasu, N.,Matsuoka, M.,Matsumura, H.,Kodama, T.,Kanai, M.,Doi, H.,Inoue, T.,Sugiyama, A. (deposition date: 2014-10-09, release date: 2015-01-21, Last modification date: 2023-11-08) |
Primary citation | Kawato, T.,Mizohata, E.,Shimizu, Y.,Meshizuka, T.,Yamamoto, T.,Takasu, N.,Matsuoka, M.,Matsumura, H.,Kodama, T.,Kanai, M.,Doi, H.,Inoue, T.,Sugiyama, A. Structure-based design and synthesis of a bivalent iminobiotin analog showing strong affinity toward a low immunogenic streptavidin mutant. Biosci.Biotechnol.Biochem., 79:640-642, 2015 Cited by PubMed: 25560769DOI: 10.1080/09168451.2014.991692 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (1.3 Å) |
Structure validation
Download full validation report