3WZP

Crystal structure of the core streptavidin mutant V21 (Y22S/N23D/S27D/Y83S/R84K/E101D/R103K/E116N) complexed with iminobiotin long tail (IMNtail) at 1.2 A resolution

Summary for 3WZP

Entry DOI	10.2210/pdb3wzp/pdb
Related	3WZN 3WZO 3WZQ
Descriptor	Streptavidin, 6-({5-[(2E,3aS,4S,6aR)-2-iminohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoyl}amino)hexanoic acid, GLYCEROL, ... (4 entities in total)
Functional Keywords	beta-barrel, biotin binding protein
Biological source	Streptomyces avidinii
Cellular location	Secreted: P22629
Total number of polymer chains	4
Total formula weight	54795.37
Authors	Kawato, T.,Mizohata, E.,Shimizu, Y.,Meshizuka, T.,Yamamoto, T.,Takasu, N.,Matsuoka, M.,Matsumura, H.,Tsumoto, K.,Kodama, T.,Kanai, M.,Doi, H.,Inoue, T.,Sugiyama, A. (deposition date: 2014-10-01, release date: 2015-02-18, Last modification date: 2023-11-08)
Primary citation	Kawato, T.,Mizohata, E.,Shimizu, Y.,Meshizuka, T.,Yamamoto, T.,Takasu, N.,Matsuoka, M.,Matsumura, H.,Kodama, T.,Kanai, M.,Doi, H.,Inoue, T.,Sugiyama, A. Structure-based design of a streptavidin mutant specific for an artificial biotin analogue. J.Biochem., 157:467-475, 2015 Cited by PubMed: 25645976 DOI: 10.1093/jb/mvv004 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.2 Å)

Structure validation