3X00

Crystal structure of the core streptavidin mutant V212 (Y22S/N23D/S27D/S45N/Y83S/R84K/E101D/R103K/E116N) complexed with bis iminobiotin long tail (Bis-IMNtail) at 1.3 A resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Streptavidin	polymer	147	15310.6	4	UniProt (P22629) Pfam (PF01382)	Streptomyces avidinii
2	E, F, H, I (A, B, C, D)	6-({5-[(2E,3aS,4S,6aR)-2-iminohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoyl}amino)hexanoic acid	non-polymer		356.5	4	Chemie (ZOF)
3	G, J (B, D)	ETHANE-1,2-DIAMINE	non-polymer		60.1	2	Chemie (EDN)
4	K, L, M, N (A, B, C, D)	water	water		18.0	442	Chemie (HOH)

Sequence modifications

A, B, C, D: 13 - 139 (UniProt: P22629)

PDB	External Database	Details
Met -1	-	expression tag
Ala 0	-	expression tag
Ser 1	-	expression tag
Met 2	-	expression tag
Thr 3	-	expression tag
Gly 4	-	expression tag
Gly 5	-	expression tag
Gln 6	-	expression tag
Gln 7	-	expression tag
Met 8	-	expression tag
Gly 9	-	expression tag
Arg 10	-	expression tag
Gly 11	-	expression tag
Ser 12	-	expression tag
Ser 22	Tyr 46	engineered mutation
Asp 23	Asn 47	engineered mutation
Asp 27	Ser 51	engineered mutation
Asn 45	Ser 69	engineered mutation
Ser 83	Tyr 107	engineered mutation
Lys 84	Arg 108	engineered mutation
Asp 101	Glu 125	engineered mutation
Lys 103	Arg 127	engineered mutation
Asn 116	Glu 140	engineered mutation
His 140	-	expression tag
His 141	-	expression tag
His 142	-	expression tag
His 143	-	expression tag
His 144	-	expression tag
His 145	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	61242.3
Non-Polymers*	Number of molecules	6
	Total formula weight	1546.1
All*	Total formula weight	62788.4

*Water molecules are not included.