Summary for 3WXM
| Entry DOI | 10.2210/pdb3wxm/pdb |
| Descriptor | Elongation factor 1-alpha, Protein pelota homolog, GUANOSINE-5'-TRIPHOSPHATE, ... (5 entities in total) |
| Functional Keywords | mrna surveillance, ribosome, translation-hydrolase complex, translation/hydrolase |
| Biological source | Aeropyrum pernix K1 More |
| Cellular location | Cytoplasm (By similarity): Q9YAV0 Cytoplasm (Potential): Q9YAZ5 |
| Total number of polymer chains | 8 |
| Total formula weight | 366722.58 |
| Authors | Kobayashi, K.,Ishitani, R.,Nureki, O. (deposition date: 2014-08-04, release date: 2014-09-03, Last modification date: 2024-03-20) |
| Primary citation | Kobayashi, K.,Kikuno, I.,Kuroha, K.,Saito, K.,Ito, K.,Ishitani, R.,Inada, T.,Nureki, O. Structural basis for mRNA surveillance by archaeal Pelota and GTP-bound EF1 alpha complex Proc.Natl.Acad.Sci.USA, 107:17575-17579, 2010 Cited by PubMed Abstract: No-go decay and nonstop decay are mRNA surveillance pathways that detect translational stalling and degrade the underlying mRNA, allowing the correct translation of the genetic code. In eukaryotes, the protein complex of Pelota (yeast Dom34) and Hbs1 translational GTPase recognizes the stalled ribosome containing the defective mRNA. Recently, we found that archaeal Pelota (aPelota) associates with archaeal elongation factor 1α (aEF1α) to act in the mRNA surveillance pathway, which accounts for the lack of an Hbs1 ortholog in archaea. Here we present the complex structure of aPelota and GTP-bound aEF1α determined at 2.3-Å resolution. The structure reveals how GTP-bound aEF1α recognizes aPelota and how aPelota in turn stabilizes the GTP form of aEF1α. Combined with the functional analysis in yeast, the present results provide structural insights into the molecular interaction between eukaryotic Pelota and Hbs1. Strikingly, the aPelota·aEF1α complex structurally resembles the tRNA·EF-Tu complex bound to the ribosome. Our findings suggest that the molecular mimicry of tRNA in the distorted "A/T state" conformation by Pelota enables the complex to efficiently detect and enter the empty A site of the stalled ribosome. PubMed: 20876129DOI: 10.1073/pnas.1009598107 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.3 Å) |
Structure validation
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