3WVJ

The crystal structure of native glycosidic hydrolase

Summary for 3WVJ

• Entry DOI: 10.2210/pdb3wvj/pdb
• Descriptor: Beta-glucanase, 2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL (3 entities in total)
• Functional Keywords: beta-1, 3-1, 4-glucanase, thermostability, industrial enzyme, pichia pastoris, a classic beta-jellyroll fold, hydrolase
• Biological source: Clostridium thermocellum
• Total number of polymer chains: 2
• Total formula weight: 51899.62

Authors

Chen, C.C.,Huang, J.W.,Zhao, P.,Ko, T.P.,Huang, C.H.,Chan, H.C.,Huang, Z.,Liu, W.,Cheng, Y.S.,Liu, J.R.,Guo, R.T. (deposition date: 2014-05-22, release date: 2015-06-24, Last modification date: 2023-11-08)

Primary citation

Chen, C.C.,Huang, J.W.,Zhao, P.,Ko, T.P.,Huang, C.H.,Chan, H.C.,Huang, Z.,Liu, W.,Cheng, Y.S.,Liu, J.R.,Guo, R.T.
Structural analyses and yeast production of the beta-1,3-1,4-glucanase catalytic module encoded by the licB gene of Clostridium thermocellum.
Enzyme.Microb.Technol., 71:1-7, 2015

PubMed Abstract: A thermophilic glycoside hydrolase family 16 (GH16) β-1,3-1,4-glucanase from Clostridium thermocellum (CtLic16A) holds great potentials in industrial applications due to its high specific activity and outstanding thermostability. In order to understand its molecular machinery, the crystal structure of CtLic16A was determined to 1.95Å resolution. The enzyme folds into a classic GH16 β-jellyroll architecture which consists of two β-sheets atop each other, with the substrate-binding cleft lying on the concave side of the inner β-sheet. Two Bis-Tris propane molecules were found in the positive and negative substrate binding sites. Structural analysis suggests that the major differences between the CtLic16A and other GH16 β-1,3-1,4-glucanase structures occur at the protein exterior. Furthermore, the high catalytic efficacy and thermal profile of the CtLic16A are preserved in the enzyme produced in Pichia pastoris, encouraging its further commercial applications.

PubMed: 25765303
DOI: 10.1016/j.enzmictec.2015.01.002

Experimental method

X-RAY DIFFRACTION (1.95 Å)