3WVJ
The crystal structure of native glycosidic hydrolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97622 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 74.450, 74.450, 182.918 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 1.950 |
| R-factor | 0.19168 |
| Rwork | 0.190 |
| R-free | 0.23249 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mac |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.258 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.480 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.053 | 0.387 |
| Number of reflections | 43722 | |
| <I/σ(I)> | 37.5 | 5.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.5 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 298 | 0.04M citric acid, 0.06M BIS-TRIS propane, pH 6.4, 18% w/v PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
