3WI3
Crystal Structure of the Sld3/Treslin domain from yeast Sld3
Summary for 3WI3
| Entry DOI | 10.2210/pdb3wi3/pdb |
| Descriptor | DNA replication regulator SLD3, SULFATE ION, 1,2-ETHANEDIOL, ... (4 entities in total) |
| Functional Keywords | replication regulator, cdc45-binding, alpha helical |
| Biological source | Saccharomyces cerevisiae (Baker's yeast) |
| Cellular location | Nucleus (By similarity): P53135 |
| Total number of polymer chains | 3 |
| Total formula weight | 104509.47 |
| Authors | Itou, H.,Araki, H.,Shirakihara, Y. (deposition date: 2013-09-05, release date: 2014-08-20, Last modification date: 2024-03-20) |
| Primary citation | Itou, H.,Muramatsu, S.,Shirakihara, Y.,Araki, H. Crystal structure of the homology domain of the eukaryotic DNA replication proteins sld3/treslin. Structure, 22:1341-1347, 2014 Cited by PubMed Abstract: The initiation of eukaryotic chromosomal DNA replication requires the formation of an active replicative helicase at the replication origins of chromosomal DNA. Yeast Sld3 and its metazoan counterpart Treslin are the hub proteins mediating protein associations critical for the helicase formation. Here, we show the crystal structure of the central domain of Sld3 that is conserved in Sld3/Treslin family of proteins. The domain consists of two segments with 12 helices and is sufficient to bind to Cdc45, the essential helicase component. The structure model of the Sld3-Cdc45 complex, which is crucial for the formation of the active helicase, is proposed. PubMed: 25126958DOI: 10.1016/j.str.2014.07.001 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.4 Å) |
Structure validation
Download full validation report
