3WI3
Crystal Structure of the Sld3/Treslin domain from yeast Sld3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-31 |
| Detector | ADSC |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.264, 92.820, 160.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.640 - 2.400 |
| R-factor | 0.219 |
| Rwork | 0.217 |
| R-free | 0.26240 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.008 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.065 | 0.400 |
| Number of reflections | 34298 | |
| <I/σ(I)> | 34.6 | 3.7 |
| Completeness [%] | 99.2 | 98 |
| Redundancy | 10.8 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 293 | PEG 4000, LiSO4, pH 8.4, vapor diffusion, hanging drop, temperature 293K |
