3UR6

1.5A resolution structure of apo Norwalk Virus Protease

3UR6 の概要

• エントリーDOI: 10.2210/pdb3ur6/pdb
• 関連するPDBエントリー: 3P99 3UR9 4DCD 4F49
• 分子名称: 3C-like protease (2 entities in total)
• 機能のキーワード: protease, norovirus, norwalk virus, antiviral inhibitors, dipeptidyl inhibitor, hydrolase
• 由来する生物種: Norovirus
• 細胞内の位置: Protein p48: Host membrane ; Single-pass membrane protein . NTPase: Host membrane ; Single- pass membrane protein . Protein p22: Host membrane ; Single-pass membrane protein : Q83883
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 40252.26

構造登録者

Lovell, S.,Battaile, K.P.,Kim, Y.,Tiew, K.C.,Mandadapu, S.R.,Alliston, K.R.,Groutas, W.C.,Chang, K.O. (登録日: 2011-11-21, 公開日: 2012-09-05, 最終更新日: 2023-09-13)

主引用文献

Kim, Y.,Lovell, S.,Tiew, K.C.,Mandadapu, S.R.,Alliston, K.R.,Battaile, K.P.,Groutas, W.C.,Chang, K.O.
Broad-Spectrum Antivirals against 3C or 3C-Like Proteases of Picornaviruses, Noroviruses, and Coronaviruses.
J.Virol., 86:11754-11762, 2012

PubMed Abstract: Phylogenetic analysis has demonstrated that some positive-sense RNA viruses can be classified into the picornavirus-like supercluster, which includes picornaviruses, caliciviruses, and coronaviruses. These viruses possess 3C or 3C-like proteases (3Cpro or 3CLpro, respectively), which contain a typical chymotrypsin-like fold and a catalytic triad (or dyad) with a Cys residue as a nucleophile. The conserved key sites of 3Cpro or 3CLpro may serve as attractive targets for the design of broad-spectrum antivirals for multiple viruses in the supercluster. We previously reported the structure-based design and synthesis of potent protease inhibitors of Norwalk virus (NV), a member of the Caliciviridae family. We report herein the broad-spectrum antiviral activities of three compounds possessing a common dipeptidyl residue with different warheads, i.e., an aldehyde (GC373), a bisulfite adduct (GC376), and an α-ketoamide (GC375), against viruses that belong to the supercluster. All compounds were highly effective against the majority of tested viruses, with half-maximal inhibitory concentrations in the high nanomolar or low micromolar range in enzyme- and/or cell-based assays and with high therapeutic indices. We also report the high-resolution X-ray cocrystal structures of NV 3CLpro-, poliovirus 3Cpro-, and transmissible gastroenteritis virus 3CLpro- GC376 inhibitor complexes, which show the compound covalently bound to a nucleophilic Cys residue in the catalytic site of the corresponding protease. We conclude that these compounds have the potential to be developed as antiviral therapeutics aimed at a single virus or multiple viruses in the picornavirus-like supercluster by targeting 3Cpro or 3CLpro.

PubMed: 22915796
DOI: 10.1128/JVI.01348-12

実験手法

X-RAY DIFFRACTION (1.5 Å)