3UDG
Structure of Deinococcus radiodurans SSB bound to ssDNA
Summary for 3UDG
Entry DOI | 10.2210/pdb3udg/pdb |
Descriptor | Single-stranded DNA-binding protein, 5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3', THYMIDINE-5'-PHOSPHATE, ... (4 entities in total) |
Functional Keywords | ssb, ob fold, beta-barrel, single-stranded dna-binding, dna binding protein-dna complex, dna binding protein/dna |
Biological source | Deinococcus radiodurans More |
Cellular location | Cytoplasm : Q9RY51 |
Total number of polymer chains | 9 |
Total formula weight | 163183.59 |
Authors | George, N.P.,Ngo, K.V.,Chitteni-Patu, S.,Norais, C.A.,Battista, J.R.,Cox, M.M.,Keck, J.L. (deposition date: 2011-10-28, release date: 2012-05-16, Last modification date: 2024-02-28) |
Primary citation | George, N.P.,Ngo, K.V.,Chitteni-Pattu, S.,Norais, C.A.,Battista, J.R.,Cox, M.M.,Keck, J.L. Structure and Cellular Dynamics of Deinococcus radiodurans Single-stranded DNA (ssDNA)-binding Protein (SSB)-DNA Complexes. J.Biol.Chem., 287:22123-22132, 2012 Cited by PubMed Abstract: The single-stranded DNA (ssDNA)-binding protein from the radiation-resistant bacterium Deinococcus radiodurans (DrSSB) functions as a homodimer in which each monomer contains two oligonucleotide-binding (OB) domains. This arrangement is exceedingly rare among bacterial SSBs, which typically form homotetramers of single-OB domain subunits. To better understand how this unusual structure influences the DNA binding and biological functions of DrSSB in D. radiodurans radiation resistance, we have examined the structure of DrSSB in complex with ssDNA and the DNA damage-dependent cellular dynamics of DrSSB. The x-ray crystal structure of the DrSSB-ssDNA complex shows that ssDNA binds to surfaces of DrSSB that are analogous to those mapped in homotetrameric SSBs, although there are distinct contacts in DrSSB that mediate species-specific ssDNA binding. Observations by electron microscopy reveal two salt-dependent ssDNA-binding modes for DrSSB that strongly resemble those of the homotetrameric Escherichia coli SSB, further supporting a shared overall DNA binding mechanism between the two classes of bacterial SSBs. In vivo, DrSSB levels are heavily induced following exposure to ionizing radiation. This accumulation is accompanied by dramatic time-dependent DrSSB cellular dynamics in which a single nucleoid-centric focus of DrSSB is observed within 1 h of irradiation but is dispersed by 3 h after irradiation. These kinetics parallel those of D. radiodurans postirradiation genome reconstitution, suggesting that DrSSB dynamics could play important organizational roles in DNA repair. PubMed: 22570477DOI: 10.1074/jbc.M112.367573 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.4 Å) |
Structure validation
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