3UDG
Structure of Deinococcus radiodurans SSB bound to ssDNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2007-05-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97869 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 161.144, 95.675, 65.726 |
| Unit cell angles | 90.00, 94.12, 90.00 |
Refinement procedure
| Resolution | 28.380 - 2.400 |
| R-factor | 0.2044 |
| Rwork | 0.201 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.727 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 5.170 | 2.400 |
| Rmerge | 0.063 | 0.038 | 0.272 |
| Number of reflections | 36689 | ||
| <I/σ(I)> | 14.1 | 46.47 | 3.11 |
| Completeness [%] | 93.9 | 98.7 | 62.6 |
| Redundancy | 3.3 | 3.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 40% PEG2KMME, 0.1 M sodium acetate pH 4.6 and 1.2 M NH4SO4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
