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3UDG

Structure of Deinococcus radiodurans SSB bound to ssDNA

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 21-ID-F
Synchrotron siteAPS
Beamline21-ID-F
Temperature [K]110
Detector technologyCCD
Collection date2007-05-15
DetectorMARMOSAIC 225 mm CCD
Wavelength(s)0.97869
Spacegroup nameC 1 2 1
Unit cell lengths161.144, 95.675, 65.726
Unit cell angles90.00, 94.12, 90.00
Refinement procedure
Resolution28.380 - 2.400
R-factor0.2044
Rwork0.201
R-free0.26100
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.013
RMSD bond angle1.727
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwarePHASER
Refinement softwareREFMAC
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]50.00050.0002.490
High resolution limit [Å]2.4005.1702.400
Rmerge0.0630.0380.272
Number of reflections36689
<I/σ(I)>14.146.473.11
Completeness [%]93.998.762.6
Redundancy3.33.42.3
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP4.629840% PEG2KMME, 0.1 M sodium acetate pH 4.6 and 1.2 M NH4SO4, VAPOR DIFFUSION, HANGING DROP, temperature 298K

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