3UDG
Structure of Deinococcus radiodurans SSB bound to ssDNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2007-05-15 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97869 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 161.144, 95.675, 65.726 |
Unit cell angles | 90.00, 94.12, 90.00 |
Refinement procedure
Resolution | 28.380 - 2.400 |
R-factor | 0.2044 |
Rwork | 0.201 |
R-free | 0.26100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.727 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 5.170 | 2.400 |
Rmerge | 0.063 | 0.038 | 0.272 |
Number of reflections | 36689 | ||
<I/σ(I)> | 14.1 | 46.47 | 3.11 |
Completeness [%] | 93.9 | 98.7 | 62.6 |
Redundancy | 3.3 | 3.4 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 40% PEG2KMME, 0.1 M sodium acetate pH 4.6 and 1.2 M NH4SO4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |