3UBV
Oxygen-bound hell's gate globin I by classical hanging drop
Summary for 3UBV
| Entry DOI | 10.2210/pdb3ubv/pdb |
| Related | 3UBC |
| Descriptor | Hemoglobin-like flavoprotein, PROTOPORPHYRIN IX CONTAINING FE, OXYGEN MOLECULE, ... (4 entities in total) |
| Functional Keywords | globin, oxygen-bound, autoxidation, oxygen, oxygen transport |
| Biological source | Methylacidiphilum infernorum V4 |
| Total number of polymer chains | 3 |
| Total formula weight | 46456.63 |
| Authors | Scudieri, D.,Belmonte, L.,Saito, J.A.,Alam, M.,Nicolini, C.,Pechkova, E. (deposition date: 2011-10-25, release date: 2012-03-28, Last modification date: 2024-02-28) |
| Primary citation | Pechkova, E.,Scudieri, D.,Belmonte, L.,Nicolini, C. Oxygen-bound Hell's gate globin I by classical versus LB nanotemplate method. J.Cell.Biochem., 8:793-794, 2012 Cited by PubMed Abstract: X-ray atomic structure of recombinant Hell's gate globin I (HGbI) from Methylacidophilum infernorum was calculated from the X-ray diffraction data of two different types of crystals: obtained by classical hanging drop and by LB nanotemplate method under the same crystallization conditions. After the accurate comparison of crystallographic parameters and electron density maps of two structures they appears to be quite similar, while the quality of the crystals grown by LB nanotemplate method was higher then of those grown by classical method. Indeed, the resolution of the LB crystal structure was 1.65 Å, while classical crystals showed only 3.2 Å resolution. Moreover, the reproducibility of this result in the case of LB crystals was much better-nine crystals from 10 gave the same structural results, while only two of 10 classical crystals were appropriate for the X-ray structure resolution. PubMed: 22415930DOI: 10.1002/jcb.24131 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.2 Å) |
Structure validation
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