3U4M

Crystal structure of ribosomal protein tthl1 in complex with 80nt 23s rna from thermus thermophilus

3U4M の概要

• エントリーDOI: 10.2210/pdb3u4m/pdb
• 関連するPDBエントリー: 1MZP 1U63 1ZHO 2HW8 2VPL 3U42
• 分子名称: 50S ribosomal protein L1, RNA (80-MER), SULFATE ION, ... (8 entities in total)
• 機能のキーワード: rossmann fold, ribosomal protein, rrna, rrna binding, ribosome, rna-rna binding protein complex, rna/rna binding protein
• 由来する生物種: Thermus thermophilus; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 52056.00

構造登録者

Gabdulkhakov, A.G.,Nevskaya, N.A.,NIkonov, S.V. (登録日: 2011-10-10, 公開日: 2012-08-08, 最終更新日: 2023-09-13)

主引用文献

Tishchenko, S.,Gabdulkhakov, A.,Nevskaya, N.,Sarskikh, A.,Kostareva, O.,Nikonova, E.,Sycheva, A.,Moshkovskii, S.,Garber, M.,Nikonov, S.
High-resolution crystal structure of the isolated ribosomal L1 stalk.
Acta Crystallogr.,Sect.D, 68:1051-1057, 2012

PubMed Abstract: The crystal structure of the isolated full-length ribosomal L1 stalk, consisting of Thermus thermophilus ribosomal protein L1 in complex with a specific 80-nucleotide fragment of 23S rRNA, has been solved for the first time at high resolution. The structure revealed details of protein-RNA interactions in the L1 stalk. Analysis of the crystal packing enabled the identification of sticky sites on the protein and the 23S rRNA which may be important for ribosome assembly and function. The structure was used to model different conformational states of the ribosome. This approach provides an insight into the roles of domain II of L1 and helix 78 of rRNA in ribosome function.

PubMed: 22868771
DOI: 10.1107/S0907444912020136

実験手法

X-RAY DIFFRACTION (2 Å)