1ZHO

The structure of a ribosomal protein L1 in complex with mRNA

Summary for 1ZHO

• Entry DOI: 10.2210/pdb1zho/pdb
• Related: 1CSJ 1DWU 1U63 1ad2 1i2a 1mzp
• Descriptor: mRNA, 50S ribosomal protein L1, POTASSIUM ION, ... (4 entities in total)
• Functional Keywords: ribosome, ribosomal protein, mrna-protein complex, l1, regulation of gene expression, rna-protein interaction, structural protein-rna complex, structural protein/rna
• Biological source: Thermus thermophilus; More
• Total number of polymer chains: 8
• Total formula weight: 148746.58

Authors

Nevskaya, N.,Tishchenko, S.,Volchkov, S.,Kljashtorny, V.,Nikonova, E.,Nikonov, O.,Nikulin, A.,Kohrer, C.,Piendl, W.,Zimmermann, R.,Stockley, P.,Garber, M.,Nikonov, S. (deposition date: 2005-04-26, release date: 2006-05-09, Last modification date: 2024-10-30)

Primary citation

Nevskaya, N.,Tishchenko, S.,Volchkov, S.,Kljashtorny, V.,Nikonova, E.,Nikonov, O.,Nikulin, A.,Kohrer, C.,Piendl, W.,Zimmermann, R.,Stockley, P.,Garber, M.,Nikonov, S.
New insights into the interaction of ribosomal protein L1 with RNA.
J.Mol.Biol., 355:747-759, 2006

PubMed Abstract: The RNA-binding ability of ribosomal protein L1 is of profound interest, since L1 has a dual function as a ribosomal structural protein that binds rRNA and as a translational repressor that binds its own mRNA. Here, we report the crystal structure at 2.6 A resolution of ribosomal protein L1 from the bacterium Thermus thermophilus in complex with a 38 nt fragment of L1 mRNA from Methanoccocus vannielii. The conformation of RNA-bound T.thermophilus L1 differs dramatically from that of the isolated protein. Analysis of four copies of the L1-mRNA complex in the crystal has shown that domain II of the protein does not contribute to mRNA-specific binding. A detailed comparison of the protein-RNA interactions in the L1-mRNA and L1-rRNA complexes identified amino acid residues of L1 crucial for recognition of its specific targets on the both RNAs. Incorporation of the structure of bacterial L1 into a model of the Escherichia coli ribosome revealed two additional contact regions for L1 on the 23S rRNA that were not identified in previous ribosome models.

PubMed: 16330048
DOI: 10.1016/j.jmb.2005.10.084

Experimental method

X-RAY DIFFRACTION (2.6 Å)