3U0S

Crystal Structure of an Enzyme Redesigned Through Multiplayer Online Gaming: CE6

Summary for 3U0S

Entry DOI	10.2210/pdb3u0s/pdb
Related	1E1A 3I1C
Descriptor	Diisopropyl-fluorophosphatase, 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID, GLYCEROL, ... (6 entities in total)
Functional Keywords	protein engineering, computer-aided design, computationally-directed design, multiplayer online gaming, crowdsourcing, foldit, diels-alder, enzyme design, active site redesign, substrate specificity, beta-propeller, helix-loop-helix, loop remodel, hydrolase, de novo protein
Biological source	Loligo vulgaris (Common European squid)
Total number of polymer chains	2
Total formula weight	76536.43
Authors	Bale, J.B.,Shen, B.W.,Stoddard, B.L. (deposition date: 2011-09-29, release date: 2012-02-01, Last modification date: 2024-02-28)
Primary citation	Eiben, C.B.,Siegel, J.B.,Bale, J.B.,Cooper, S.,Khatib, F.,Shen, B.W.,Players, F.,Stoddard, B.L.,Popovic, Z.,Baker, D. Increased Diels-Alderase activity through backbone remodeling guided by Foldit players. Nat.Biotechnol., 30:190-192, 2012 Cited by PubMed: 22267011 DOI: 10.1038/nbt.2109 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.6 Å)

Structure validation