3U0S
Crystal Structure of an Enzyme Redesigned Through Multiplayer Online Gaming: CE6
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Diisopropyl-fluorophosphatase | polymer | 337 | 37442.4 | 2 | UniProt (Q7SIG4) Pfam (PF08450) | Loligo vulgaris (Common European squid) | DFPase |
| 2 | C, J (A, B) | 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID | non-polymer | 238.3 | 2 | Chemie (EPE) | |||
| 3 | D, E, F, K, L... (A, B) | GLYCEROL | non-polymer | 92.1 | 6 | Chemie (GOL) | |||
| 4 | G, H, I, P, Q... (A, B) | SULFATE ION | non-polymer | 96.1 | 6 | Chemie (SO4) | |||
| 5 | N, O (B) | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) | |||
| 6 | S, T (A, B) | water | water | 18.0 | 240 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 327 (UniProt: Q7SIG4)
| PDB | External Database | Details |
|---|---|---|
| Thr 21 | Glu 21 | engineered mutation |
| Ser 36 | - | SEE REMARK 999 |
| Leu 38 | Glu 37 | SEE REMARK 999 |
| Ser 39 | Val 38 | SEE REMARK 999 |
| Ala 41 | Val 40 | SEE REMARK 999 |
| Leu 42 | Asn 41 | SEE REMARK 999 |
| Thr 43 | Gly 42 | SEE REMARK 999 |
| Ala 45 | - | SEE REMARK 999 |
| Asn 46 | - | SEE REMARK 999 |
| Ser 47 | - | SEE REMARK 999 |
| Ala 49 | - | SEE REMARK 999 |
| Glu 50 | - | SEE REMARK 999 |
| Ala 51 | - | SEE REMARK 999 |
| Tyr 52 | - | SEE REMARK 999 |
| Lys 53 | - | SEE REMARK 999 |
| Ala 54 | - | SEE REMARK 999 |
| Ser 55 | - | SEE REMARK 999 |
| Arg 56 | - | SEE REMARK 999 |
| Gly 57 | - | SEE REMARK 999 |
| Ser 85 | Ile 72 | engineered mutation |
| Ile 87 | Ala 74 | engineered mutation |
| Ala 133 | Asn 120 | engineered mutation |
| Tyr 134 | Asp 121 | engineered mutation |
| Phe 157 | Tyr 144 | engineered mutation |
| Ile 159 | Arg 146 | engineered mutation |
| Leu 161 | Met 148 | engineered mutation |
| Arg 162 | Gln 149 | engineered mutation |
| Cys 186 | Phe 173 | engineered mutation |
| Ala 188 | Asn 175 | engineered mutation |
| Gln 208 | Thr 195 | engineered mutation |
| Lys 238 | Glu 225 | engineered mutation |
| Ala 242 | Asp 229 | engineered mutation |
| Ala 284 | Ser 271 | engineered mutation |
| Gly 328 | - | expression tag |
| Ser 329 | - | expression tag |
| Leu 330 | - | expression tag |
| Glu 331 | - | expression tag |
| His 332 | - | expression tag |
| His 333 | - | expression tag |
| His 334 | - | expression tag |
| His 335 | - | expression tag |
| His 336 | - | expression tag |
| His 337 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 74884.9 | |
| Non-Polymers* | Number of molecules | 16 |
| Total formula weight | 1651.5 | |
| All* | Total formula weight | 76536.4 |
