3TW4

Crystal Structure of Human Septin 7 GTPase Domain

Summary for 3TW4

• Entry DOI: 10.2210/pdb3tw4/pdb
• Descriptor: Septin-7, GUANOSINE-5'-DIPHOSPHATE (2 entities in total)
• Functional Keywords: gtpase, septins, cell cycle
• Biological source: Homo sapiens (human)
• Cellular location: Cytoplasm : Q16181
• Total number of polymer chains: 2
• Total formula weight: 63163.77

Authors

Serrao, V.H.B.,Alessandro, F.,Pereira, H.M.,Thiemann, O.T.,Garratt, R.C. (deposition date: 2011-09-21, release date: 2011-11-23, Last modification date: 2023-09-13)

Primary citation

Serrao, V.H.,Alessandro, F.,Caldas, V.E.,Marcal, R.L.,D'Muniz Pereira, H.,Thiemann, O.H.,Garratt, R.C.
Promiscuous interactions of human septins: The GTP binding domain of SEPT7 forms filaments within the crystal.
Febs Lett., 585:3868-3873, 2011

PubMed Abstract: We describe the purification, crystallization and structure for the GTP-binding domain of human septin 7 (SEPT7G). We show that it forms filaments within the crystal lattice which employ both the G and NC interfaces, similar to those seen in the hetero-filament of SEPT2/6/7. The NC interface is considered promiscuous as it is absent from the hetero-filament. Such promiscuity could provide the potential for permuting monomers along a filament in order to generate diversity in hetero-polymers. On the other hand, our results suggest that the G and NC interfaces may be necessary but insufficient for determining correct hetero-filament assembly.

PubMed: 22064074
DOI: 10.1016/j.febslet.2011.10.043

Experimental method

X-RAY DIFFRACTION (3.35 Å)