3TW4
Crystal Structure of Human Septin 7 GTPase Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-09-09 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97627 |
| Spacegroup name | P 61 |
| Unit cell lengths | 82.510, 82.510, 210.910 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.420 - 3.350 |
| R-factor | 0.2927 |
| Rwork | 0.289 |
| R-free | 0.36520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qnr |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.544 |
| Data reduction software | MOSFLM |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 42.420 | 3.550 | |
| High resolution limit [Å] | 3.350 | 9.800 | 3.350 |
| Rmerge | 0.049 | 0.012 | 0.660 |
| Number of reflections | 11558 | 470 | 1825 |
| <I/σ(I)> | 15.1 | 66.17 | 1.59 |
| Completeness [%] | 98.7 | 97.7 | 97.8 |
| Redundancy | 3.01 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 291 | 100mM Tris pH 8.4; 16% Glycerol; 1.5M Amonium Sulphate; 500mM GDP; 12.5% Isopropanol and 7mg/mL SETPT7G, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
