3TIS
Crystal structures of yrdA from Escherichia coli, a homologous protein of gamma-class carbonic anhydrases, show possible allosteric conformations
Summary for 3TIS
| Entry DOI | 10.2210/pdb3tis/pdb |
| Related | 3TIO |
| Descriptor | Protein YrdA, ZINC ION (3 entities in total) |
| Functional Keywords | carbonic anhydrase (ca), zn biding, phosphate binding, transferase |
| Biological source | Escherichia coli |
| Total number of polymer chains | 3 |
| Total formula weight | 60546.49 |
| Authors | Park, H.M.,Chio, J.W.,Lee, J.E.,Jung, J.H.,Kim, B.Y.,Kim, J.S. (deposition date: 2011-08-21, release date: 2012-08-01, Last modification date: 2024-03-20) |
| Primary citation | Park, H.M.,Park, J.H.,Choi, J.W.,Lee, J.E.,Kim, B.Y.,Jung, C.H.,Kim, J.S. Structures of the gamma-class carbonic anhydrase homologue YrdA suggest a possible allosteric switch Acta Crystallogr.,Sect.D, 68:920-926, 2012 Cited by PubMed Abstract: The YrdA protein shows high sequence similarity to γ-class carbonic anhydrase (γ-CA) proteins and is classified as part of the γ-CA protein family. However, its function has not been fully elucidated as it lacks several of the conserved residues that are considered to be necessary for γ-CA catalysis. Interestingly, a homologue of γ-CA from Methanosarcina thermophila and a β-carboxysomal γ-CA from a β-cyanobacterium have shown that these catalytic residues are not always conserved in γ-CAs. The crystal structure of YrdA from Escherichia coli (ecYrdA) is reported here in two crystallographic forms. The overall structure of ecYrdA is also similar to those of the γ-CAs. One loop around the putative catalytic site shows a number of alternative conformations. A His residue (His70) on this loop coordinates with, or is reoriented from, the catalytic Zn(2+) ion; this is similar to the conformations mediated by an Asp residue on the catalytic loops of β-CA proteins. One Trp residue (Trp171) also adopts two alternative conformations that may be related to the spatial positions of the catalytic loop. Even though significant CA activity could not be detected using purified ecYrdA, these structural features have potential functional implications for γ-CA-related proteins. PubMed: 22868757DOI: 10.1107/S0907444912017210 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.3 Å) |
Structure validation
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