3TIS
Crystal structures of yrdA from Escherichia coli, a homologous protein of gamma-class carbonic anhydrases, show possible allosteric conformations
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 6C1 |
Synchrotron site | PAL/PLS |
Beamline | 6C1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-12 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 63 |
Unit cell lengths | 96.793, 96.793, 87.398 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.300 |
R-factor | 0.1798 |
Rwork | 0.180 |
R-free | 0.24220 |
Structure solution method | MIR |
RMSD bond length | 0.005 |
RMSD bond angle | 1.273 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 20615 | |
Completeness [%] | 99.2 | 99.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 20% (w/v) polyethyleneglycol Monomethyl Ether 2000, 0.15M (NH4)2SO4, 0.1M Sodium Acetate , pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |