3T94

Crystal structure of 5'-deoxy-5'-methylthioadenosine phosphorylase (MTAP) II complexed with 5'-deoxy-5'-methylthioadenosine and sulfate

Summary for 3T94

• Entry DOI: 10.2210/pdb3t94/pdb
• Related: 2A8Y
• Descriptor: 5'-methylthioadenosine phosphorylase (MtaP), 5'-DEOXY-5'-METHYLTHIOADENOSINE, SULFATE ION, ... (4 entities in total)
• Functional Keywords: alpha/beta, beta barrel, nucleoside phosphorylase, transferase
• Biological source: Sulfolobus solfataricus
• Total number of polymer chains: 6
• Total formula weight: 183907.47

Authors

Zhang, Y.,Ealick, S.E. (deposition date: 2011-08-02, release date: 2011-08-24, Last modification date: 2024-11-27)

Primary citation

Zhang, Y.,Zwart, P.H.,Ealick, S.E.
A corrected space group for Sulfolobus sulfataricus 5'-deoxy-5'-methylthioadenosine phosphorylase II.
Acta Crystallogr.,Sect.D, 68:249-252, 2012

PubMed Abstract: 5'-deoxy-5'-methylthioadenosine phosphorylase (MTAP) catalyzes the phosphorolytic cleavage of 5'-deoxy-5'-methylthioadenosine (MTA), a byproduct of polyamine biosynthesis. The Sulfolobus sulfataricus genome encodes two MTAPs. SsMTAP I has broad substrate specifity, accepting guanosine, inosine, adenosine and MTA, while SsMTAP II is specific for MTA. SsMTAP I forms a donut-shaped hexamer, while SsMTAP II is a hexamer formed from trimers packed face to face. The structure of SsMTAP II was originally determined in space group P1 (PDB entry 2a8y) and showed R32 pseudosymmetry. Post-analysis using phenix.xtriage showed that the correct space group is C2. Here, the structure refined in space group C2 is reported and the factors that initially led to the incorrect space-group assignment are discussed.

PubMed: 22349226
DOI: 10.1107/S0907444911051699

Experimental method

X-RAY DIFFRACTION (1.452 Å)