3T1F

Crystal structure of the mouse CD1d-Glc-DAG-s2 complex

Summary for 3T1F

• Entry DOI: 10.2210/pdb3t1f/pdb
• Descriptor: Antigen-presenting glycoprotein CD1d1, Beta-2-microglobulin, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (7 entities in total)
• Functional Keywords: antigen presentation, glycolipid, nkt cells, immune system
• Biological source: Mus musculus (mouse); More
• Total number of polymer chains: 2
• Total formula weight: 46377.39

Authors

Girardi, E.,Zajonc, D.M. (deposition date: 2011-07-21, release date: 2011-09-21, Last modification date: 2024-11-27)

Primary citation

Kinjo, Y.,Illarionov, P.,Vela, J.L.,Pei, B.,Girardi, E.,Li, X.,Li, Y.,Imamura, M.,Kaneko, Y.,Okawara, A.,Miyazaki, Y.,Gomez-Velasco, A.,Rogers, P.,Dahesh, S.,Uchiyama, S.,Khurana, A.,Kawahara, K.,Yesilkaya, H.,Andrew, P.W.,Wong, C.H.,Kawakami, K.,Nizet, V.,Besra, G.S.,Tsuji, M.,Zajonc, D.M.,Kronenberg, M.
Invariant natural killer T cells recognize glycolipids from pathogenic Gram-positive bacteria.
Nat.Immunol., 12:966-974, 2011

PubMed Abstract: Natural killer T cells (NKT cells) recognize glycolipid antigens presented by CD1d. These cells express an evolutionarily conserved, invariant T cell antigen receptor (TCR), but the forces that drive TCR conservation have remained uncertain. Here we show that NKT cells recognized diacylglycerol-containing glycolipids from Streptococcus pneumoniae, the leading cause of community-acquired pneumonia, and group B Streptococcus, which causes neonatal sepsis and meningitis. Furthermore, CD1d-dependent responses by NKT cells were required for activation and host protection. The glycolipid response was dependent on vaccenic acid, which is present in low concentrations in mammalian cells. Our results show how microbial lipids position the sugar for recognition by the invariant TCR and, most notably, extend the range of microbes recognized by this conserved TCR to several clinically important bacteria.

PubMed: 21892173
DOI: 10.1038/ni.2096

Experimental method

X-RAY DIFFRACTION (1.7 Å)