3SV3

Crystal structure of the large fragment of DNA polymerase I from Thermus Aquaticus in a closed ternary complex with the artificial base pair dNaM-d5SICSTP

3SV3 の概要

• エントリーDOI: 10.2210/pdb3sv3/pdb
• 関連するPDBエントリー: 3KTQ 3RTV 3SV4 3SYZ 3SZ2
• 分子名称: DNA polymerase I, thermostable, (5'-D(*GP*AP*CP*CP*AP*CP*GP*GP*CP*GP*CP*(DOC))-3'), (5'-D(*AP*AP*AP*(BMN)P*GP*GP*CP*GP*CP*CP*GP*TP*GP*GP*TP*C)-3'), ... (8 entities in total)
• 機能のキーワード: dna polymerase, artificial base pair, transferase-dna complex, transferase/dna
• 由来する生物種: Thermus aquaticus; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 71814.53

構造登録者

Betz, K.,Diederichs, K.,Marx, A. (登録日: 2011-07-12, 公開日: 2012-06-06, 最終更新日: 2023-09-13)

主引用文献

Betz, K.,Malyshev, D.A.,Lavergne, T.,Welte, W.,Diederichs, K.,Dwyer, T.J.,Ordoukhanian, P.,Romesberg, F.E.,Marx, A.
KlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry.
Nat.Chem.Biol., 8:612-614, 2012

PubMed Abstract: Many candidate unnatural DNA base pairs have been developed, but some of the best-replicated pairs adopt intercalated structures in free DNA that are difficult to reconcile with known mechanisms of polymerase recognition. Here we present crystal structures of KlenTaq DNA polymerase at different stages of replication for one such pair, dNaM-d5SICS, and show that efficient replication results from the polymerase itself, inducing the required natural-like structure.

PubMed: 22660438
DOI: 10.1038/nchembio.966

実験手法

X-RAY DIFFRACTION (2.1 Å)