3SV3
Crystal structure of the large fragment of DNA polymerase I from Thermus Aquaticus in a closed ternary complex with the artificial base pair dNaM-d5SICSTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-12-17 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 108.545, 108.545, 90.978 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.001 - 2.100 |
| R-factor | 0.1698 |
| Rwork | 0.167 |
| R-free | 0.21540 |
| Starting model (for MR) | 3m8s |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.144 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX (1.7.1_743) |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 2.230 |
| High resolution limit [Å] | 2.100 | 2.100 |
| <I/σ(I)> | 12.41 | 2.33 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 9.98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 13% PEG 8000, 100mM NH4Cl, 0.1M MgFormate, 0.1M Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
