3SNP

Crystal structure analysis of iron regulatory protein 1 in complex with ferritin H IRE RNA

Replaces:  2IPY

Summary for 3SNP

• Entry DOI: 10.2210/pdb3snp/pdb
• Related: 2IPY 3SN2
• Descriptor: Cytoplasmic aconitate hydratase, ferritin H IRE RNA (3 entities in total)
• Functional Keywords: rna binding, iron sulfur cluster binding, phosphorylation, lyase-rna complex, lyase/rna
• Biological source: Oryctolagus cuniculus (rabbit)
• Cellular location: Cytoplasm: Q01059
• Total number of polymer chains: 4
• Total formula weight: 220690.82

Authors

Volz, K.,Selezneva, A.I.,Walden, W.E. (deposition date: 2011-06-29, release date: 2011-07-27, Last modification date: 2023-09-13)

Primary citation

Walden, W.E.,Selezneva, A.I.,Dupuy, J.,Volbeda, A.,Fontecilla-Camps, J.C.,Theil, E.C.,Volz, K.
Structure of dual function iron regulatory protein 1 complexed with ferritin IRE-RNA.
Science, 314:1903-1908, 2006

PubMed Abstract: Iron regulatory protein 1 (IRP1) binds iron-responsive elements (IREs) in messenger RNAs (mRNAs), to repress translation or degradation, or binds an iron-sulfur cluster, to become a cytosolic aconitase enzyme. The 2.8 angstrom resolution crystal structure of the IRP1:ferritin H IRE complex shows an open protein conformation compared with that of cytosolic aconitase. The extended, L-shaped IRP1 molecule embraces the IRE stem-loop through interactions at two sites separated by approximately 30 angstroms, each involving about a dozen protein:RNA bonds. Extensive conformational changes related to binding the IRE or an iron-sulfur cluster explain the alternate functions of IRP1 as an mRNA regulator or enzyme.

PubMed: 17185597
DOI: 10.1126/science.1133116

Experimental method

X-RAY DIFFRACTION (2.8 Å)