3SMR

Crystal structure of human WD repeat domain 5 with compound

Summary for 3SMR

• Entry DOI: 10.2210/pdb3smr/pdb
• Descriptor: WD repeat-containing protein 5, 2-chloro-N-[2-(4-methylpiperazin-1-yl)-5-nitrophenyl]benzamide, 1,2-ETHANEDIOL, ... (5 entities in total)
• Functional Keywords: wdr5, sgc, structural genomics, structural genomics consortium, wd repeat domain 5, transcription
• Biological source: Homo sapiens (human)
• Cellular location: Nucleus : P61964
• Total number of polymer chains: 4
• Total formula weight: 140086.40

Authors

Dong, A.,Dombrovski, L.,Wasney, G.A.,Tempel, W.,Senisterra, G.,Bolshan, Y.,Smil, D.,Nguyen, K.T.,Hajian, T.,Poda, G.,Al-Awar, R.,Bountra, C.,Weigelt, J.,Edwards, A.M.,Brown, P.J.,Schapira, M.,Arrowsmith, C.H.,Vedadi, M.,Wu, H.,Structural Genomics Consortium (SGC) (deposition date: 2011-06-28, release date: 2011-08-31, Last modification date: 2023-09-13)

Primary citation

Senisterra, G.,Wu, H.,Allali-Hassani, A.,Wasney, G.A.,Barsyte-Lovejoy, D.,Dombrovski, L.,Dong, A.,Nguyen, K.T.,Smil, D.,Bolshan, Y.,Hajian, T.,He, H.,Seitova, A.,Chau, I.,Li, F.,Poda, G.,Couture, J.F.,Brown, P.J.,Al-Awar, R.,Schapira, M.,Arrowsmith, C.H.,Vedadi, M.
Small-molecule inhibition of MLL activity by disruption of its interaction with WDR5.
Biochem. J., 449:151-159, 2013

PubMed Abstract: WDR5 (WD40 repeat protein 5) is an essential component of the human trithorax-like family of SET1 [Su(var)3-9 enhancer-of-zeste trithorax 1] methyltransferase complexes that carry out trimethylation of histone 3 Lys4 (H3K4me3), play key roles in development and are abnormally expressed in many cancers. In the present study, we show that the interaction between WDR5 and peptides from the catalytic domain of MLL (mixed-lineage leukaemia protein) (KMT2) can be antagonized with a small molecule. Structural and biophysical analysis show that this antagonist binds in the WDR5 peptide-binding pocket with a Kd of 450 nM and inhibits the catalytic activity of the MLL core complex in vitro. The degree of inhibition was enhanced at lower protein concentrations consistent with a role for WDR5 in directly stabilizing the MLL multiprotein complex. Our data demonstrate inhibition of an important protein-protein interaction and form the basis for further development of inhibitors of WDR5-dependent enzymes implicated in MLL-rearranged leukaemias or other cancers.

PubMed: 22989411
DOI: 10.1042/BJ20121280

Experimental method

X-RAY DIFFRACTION (1.82 Å)