3SMR
Crystal structure of human WD repeat domain 5 with compound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-06-10 |
Detector | RIGAKU RAXIS HTC |
Wavelength(s) | 1.54 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 204.171, 93.467, 64.786 |
Unit cell angles | 90.00, 107.24, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.820 |
R-factor | 0.17759 |
Rwork | 0.177 |
R-free | 0.20612 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2o9k |
RMSD bond length | 0.008 |
RMSD bond angle | 1.134 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.850 |
High resolution limit [Å] | 1.820 | 1.820 |
Number of reflections | 104206 | |
<I/σ(I)> | 25 | 2.12 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.2 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 297 | 24% PEG3350,9.2M NH4 Acetate, 0.1 Bis Tris pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |