3SHF

Crystal structure of the R265S mutant of full-length murine Apaf-1

3SHF の概要

• エントリーDOI: 10.2210/pdb3shf/pdb
• 関連するPDBエントリー: 3sfz
• 分子名称: Apoptotic peptidase activating factor 1, ADENOSINE-5'-DIPHOSPHATE, GAMMA-BUTYROLACTONE, ... (4 entities in total)
• 機能のキーワード: tandem beta-propeller, apoptosis, cytochrome c, adenine nucleotide, procaspase-9, cytosol
• 由来する生物種: Mus musculus (mouse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 142458.61

構造登録者

Eschenburg, S.,Reubold, T.F. (登録日: 2011-06-16, 公開日: 2011-08-24, 最終更新日: 2024-11-06)

主引用文献

Reubold, T.F.,Wohlgemuth, S.,Eschenburg, S.
Crystal structure of full-length Apaf-1: how the death signal is relayed in the mitochondrial pathway of apoptosis.
Structure, 19:1074-1083, 2011

PubMed Abstract: The apoptotic protease-activating factor 1 (Apaf-1) relays the death signal in the mitochondrial pathway of apoptosis. Apaf-1 oligomerizes on binding of mitochondrially released cytochrome c into the heptameric apoptosome complex to ignite the downstream cascade of caspases. Here, we present the 3.0 Å crystal structure of full-length murine Apaf-1 in the absence of cytochrome c. The structure shows how the mammalian death switch is kept in its "off" position. By comparing the off state with a recent cryo-electron microscopy derived model of Apaf-1 in its apoptosomal conformation, we depict the molecular events that transform Apaf-1 from autoinhibited monomer to a building block of the caspase-activating apoptosome. Moreover, we have solved the crystal structure of the R265S mutant of full-length murine Apaf-1 in the absence of cytochrome c to 3.55 Å resolution and we show that proper function of Apaf-1 relies on R265 in the vicinity of the bound nucleotide.

PubMed: 21827944
DOI: 10.1016/j.str.2011.05.013

実験手法

X-RAY DIFFRACTION (3.55 Å)