3SDD
Crystal structure of autoreactive-Valpha14-Vbeta6 NKT TCR in complex with CD1d-beta-lactosylceramide
Summary for 3SDD
| Entry DOI | 10.2210/pdb3sdd/pdb |
| Related | 3SCM 3SDA 3SDC 3SDX |
| Descriptor | Antigen-presenting glycoprotein CD1d1, Beta-2-microglobulin, NKT TCR Valpha14 chain, ... (8 entities in total) |
| Functional Keywords | cd1d, autoimmunity, self-recognition, nkt, immune system |
| Biological source | Mus musculus (mouse) More |
| Total number of polymer chains | 4 |
| Total formula weight | 98550.61 |
| Authors | Clarke, A.J.,Rossjohn, J. (deposition date: 2011-06-09, release date: 2011-10-05, Last modification date: 2024-10-30) |
| Primary citation | Pellicci, D.G.,Clarke, A.J.,Patel, O.,Mallevaey, T.,Beddoe, T.,Le Nours, J.,Uldrich, A.P.,McCluskey, J.,Besra, G.S.,Porcelli, S.A.,Gapin, L.,Godfrey, D.I.,Rossjohn, J. Recognition of beta-linked self glycolipids mediated by natural killer T cell antigen receptors Nat.Immunol., 12:827-833, 2011 Cited by PubMed Abstract: The most potent foreign antigens for natural killer T cells (NKT cells) are α-linked glycolipids, whereas NKT cell self-reactivity involves weaker recognition of structurally distinct β-linked glycolipid antigens. Here we provide the mechanism for the autoreactivity of T cell antigen receptors (TCRs) on NKT cells to the mono- and tri-glycosylated β-linked agonists β-galactosylceramide (β-GalCer) and isoglobotrihexosylceramide (iGb3), respectively. In binding these disparate antigens, the NKT cell TCRs docked onto CD1d similarly, achieving this by flattening the conformation of the β-linked ligands regardless of the size of the glycosyl head group. Unexpectedly, the antigenicity of iGb3 was attributable to its terminal sugar group making compensatory interactions with CD1d. Thus, the NKT cell TCR molds the β-linked self ligands to resemble the conformation of foreign α-linked ligands, which shows that induced-fit molecular mimicry can underpin the self-reactivity of NKT cell TCRs to β-linked antigens. PubMed: 21804559DOI: 10.1038/ni.2076 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3 Å) |
Structure validation
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