3SA0

Complex of ERK2 with norathyriol

Summary for 3SA0

• Entry DOI: 10.2210/pdb3sa0/pdb
• Related: 1TVO
• Descriptor: Mitogen-activated protein kinase 1, norathyriol, SULFATE ION, ... (4 entities in total)
• Functional Keywords: norathyriol complex, signal-regulated kinase, transferase-transferase inhibitor complex, transferase/transferase inhibitor
• Biological source: Homo sapiens (human)
• Cellular location: Nucleus: P28482
• Total number of polymer chains: 1
• Total formula weight: 42029.27

Authors

Kurinov, I.,Malakhova, M. (deposition date: 2011-06-02, release date: 2011-12-14, Last modification date: 2024-10-30)

Primary citation

Li, J.,Malakhova, M.,Mottamal, M.,Reddy, K.,Kurinov, I.,Carper, A.,Langfald, A.,Oi, N.,Kim, M.O.,Zhu, F.,Sosa, C.P.,Zhou, K.,Bode, A.M.,Dong, Z.
Norathyriol Suppresses Skin Cancers Induced by Solar Ultraviolet Radiation by Targeting ERK Kinases.
Cancer Res., 72:260-270, 2012

PubMed Abstract: Ultraviolet (UV) irradiation is the leading factor in the development of skin cancer, prompting great interest in chemopreventive agents for this disease. In this study, we report the discovery of norathyriol, a plant-derived chemopreventive compound identified through an in silico virtual screening of the Chinese Medicine Library. Norathyriol is a metabolite of mangiferin found in mango, Hypericum elegans, and Tripterospermum lanceolatum and is known to have anticancer activity. Mechanistic investigations determined that norathyriol acted as an inhibitor of extracellular signal-regulated kinase (ERK)1/2 activity to attenuate UVB-induced phosphorylation in mitogen-activated protein kinases signaling cascades. We confirmed the direct and specific binding of norathyriol with ERK2 through a cocrystal structural analysis. The xanthone moiety in norathyriol acted as an adenine mimetic to anchor the compound by hydrogen bonds to the hinge region of the protein ATP-binding site on ERK2. Norathyriol inhibited in vitro cell growth in mouse skin epidermal JB6 P+ cells at the level of G(2)-M phase arrest. In mouse skin tumorigenesis assays, norathyriol significantly suppressed solar UV-induced skin carcinogenesis. Further analysis indicated that norathyriol mediates its chemopreventive activity by inhibiting the ERK-dependent activity of transcriptional factors AP-1 and NF-κB during UV-induced skin carcinogenesis. Taken together, our results identify norathyriol as a safe new chemopreventive agent that is highly effective against development of UV-induced skin cancer.

PubMed: 22084399
DOI: 10.1158/0008-5472.CAN-11-2596

Experimental method

X-RAY DIFFRACTION (1.5947 Å)