3S3X

Structure of chicken acid-sensing ion channel 1 AT 3.0 A resolution in complex with psalmotoxin

Summary for 3S3X

• Entry DOI: 10.2210/pdb3s3x/pdb
• Related: 3S3W
• Descriptor: Amiloride-sensitive cation channel 2, neuronal, Psalmotoxin-1, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (7 entities in total)
• Functional Keywords: acid-sensing, ion channel, membrane protein, sodium channel, cell membrane, glycoprotein, ion transport, membrane, sodium transport, transmembrane, transport, transport protein, toxin, cysteine knot
• Biological source: Gallus gallus (bantam,chickens); More
• Total number of polymer chains: 6
• Total formula weight: 172443.85

Authors

Dawson, R.J.P.,Benz, J.,Stohler, P.,Tetaz, T.,Joseph, C.,Huber, S.,Schmid, G.,Huegin, D.,Pflimlin, P.,Trube, G.,Rudolph, M.G.,Hennig, M.,Ruf, A. (deposition date: 2011-05-18, release date: 2012-05-23, Last modification date: 2024-11-06)

Primary citation

Dawson, R.J.,Benz, J.,Stohler, P.,Tetaz, T.,Joseph, C.,Huber, S.,Schmid, G.,Hugin, D.,Pflimlin, P.,Trube, G.,Rudolph, M.G.,Hennig, M.,Ruf, A.
Structure of the Acid-sensing ion channel 1 in complex with the gating modifier Psalmotoxin 1.
Nat Commun, 3:936-936, 2012

PubMed Abstract: Venom-derived peptide toxins can modify the gating characteristics of excitatory channels in neurons. How they bind and interfere with the flow of ions without directly blocking the ion permeation pathway remains elusive. Here we report the crystal structure of the trimeric chicken Acid-sensing ion channel 1 in complex with the highly selective gating modifier Psalmotoxin 1 at 3.0 Å resolution. The structure reveals the molecular interactions of three toxin molecules binding at the proton-sensitive acidic pockets of Acid-sensing ion channel 1 and electron density consistent with a cation trapped in the central vestibule above the ion pathway. A hydrophobic patch and a basic cluster are the key structural elements of Psalmotoxin 1 binding, locking two separate regulatory regions in their relative, desensitized-like arrangement. Our results provide a general concept for gating modifier toxin binding suggesting that both surface motifs are required to modify the gating characteristics of an ion channel.

PubMed: 22760635
DOI: 10.1038/ncomms1917

Experimental method

X-RAY DIFFRACTION (2.99 Å)