3S3X

Structure of chicken acid-sensing ion channel 1 AT 3.0 A resolution in complex with psalmotoxin

3S3X の概要

• エントリーDOI: 10.2210/pdb3s3x/pdb
• 関連するPDBエントリー: 3S3W
• 分子名称: Amiloride-sensitive cation channel 2, neuronal, Psalmotoxin-1, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (7 entities in total)
• 機能のキーワード: acid-sensing, ion channel, membrane protein, sodium channel, cell membrane, glycoprotein, ion transport, membrane, sodium transport, transmembrane, transport, transport protein, toxin, cysteine knot
• 由来する生物種: Gallus gallus (bantam,chickens); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 172443.85

構造登録者

Dawson, R.J.P.,Benz, J.,Stohler, P.,Tetaz, T.,Joseph, C.,Huber, S.,Schmid, G.,Huegin, D.,Pflimlin, P.,Trube, G.,Rudolph, M.G.,Hennig, M.,Ruf, A. (登録日: 2011-05-18, 公開日: 2012-05-23, 最終更新日: 2024-11-06)

主引用文献

Dawson, R.J.,Benz, J.,Stohler, P.,Tetaz, T.,Joseph, C.,Huber, S.,Schmid, G.,Hugin, D.,Pflimlin, P.,Trube, G.,Rudolph, M.G.,Hennig, M.,Ruf, A.
Structure of the Acid-sensing ion channel 1 in complex with the gating modifier Psalmotoxin 1.
Nat Commun, 3:936-936, 2012

PubMed Abstract: Venom-derived peptide toxins can modify the gating characteristics of excitatory channels in neurons. How they bind and interfere with the flow of ions without directly blocking the ion permeation pathway remains elusive. Here we report the crystal structure of the trimeric chicken Acid-sensing ion channel 1 in complex with the highly selective gating modifier Psalmotoxin 1 at 3.0 Å resolution. The structure reveals the molecular interactions of three toxin molecules binding at the proton-sensitive acidic pockets of Acid-sensing ion channel 1 and electron density consistent with a cation trapped in the central vestibule above the ion pathway. A hydrophobic patch and a basic cluster are the key structural elements of Psalmotoxin 1 binding, locking two separate regulatory regions in their relative, desensitized-like arrangement. Our results provide a general concept for gating modifier toxin binding suggesting that both surface motifs are required to modify the gating characteristics of an ion channel.

PubMed: 22760635
DOI: 10.1038/ncomms1917

実験手法

X-RAY DIFFRACTION (2.99 Å)