3RUX

Crystal structure of biotin-protein ligase BirA from Mycobacterium tuberculosis in complex with an acylsulfamide bisubstrate inhibitor

Summary for 3RUX

• Entry DOI: 10.2210/pdb3rux/pdb
• Related: 2CGH 3L1A 3L2Z
• Descriptor: BirA bifunctional protein, 5'-deoxy-5'-[({5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoyl}sulfamoyl)amino]adenosine (3 entities in total)
• Functional Keywords: biotin-protein ligase, ligase-ligase inhibitor complex, ligase/ligase inhibitor
• Biological source: Mycobacterium tuberculosis
• Total number of polymer chains: 2
• Total formula weight: 58212.12

Authors

Geders, T.W.,Finzel, B.C. (deposition date: 2011-05-05, release date: 2011-12-14, Last modification date: 2024-02-28)

Primary citation

Duckworth, B.P.,Geders, T.W.,Tiwari, D.,Boshoff, H.I.,Sibbald, P.A.,Barry, C.E.,Schnappinger, D.,Finzel, B.C.,Aldrich, C.C.
Bisubstrate Adenylation Inhibitors of Biotin Protein Ligase from Mycobacterium tuberculosis.
Chem.Biol., 18:1432-1441, 2011

PubMed Abstract: The mycobacterial biotin protein ligase (MtBPL) globally regulates lipid metabolism in Mtb through the posttranslational biotinylation of acyl coenzyme A carboxylases involved in lipid biosynthesis that catalyze the first step in fatty acid biosynthesis and pyruvate coenzyme A carboxylase, a gluconeogenic enzyme vital for lipid catabolism. Here we describe the design, development, and evaluation of a rationally designed bisubstrate inhibitor of MtBPL. This inhibitor displays potent subnanomolar enzyme inhibition and antitubercular activity against multidrug resistant and extensively drug resistant Mtb strains. We show that the inhibitor decreases in vivo protein biotinylation of key enzymes involved in fatty acid biosynthesis and that the antibacterial activity is MtBPL dependent. Additionally, the gene encoding BPL was found to be essential in M. smegmatis. Finally, the X-ray cocrystal structure of inhibitor bound MtBPL was solved providing detailed insight for further structure-activity analysis. Collectively, these data suggest that MtBPL is a promising target for further antitubercular therapeutic development.

PubMed: 22118677
DOI: 10.1016/j.chembiol.2011.08.013

Experimental method

X-RAY DIFFRACTION (1.7 Å)