3RSM

Crystal structure of S108C mutant of PMM/PGM

Summary for 3RSM

• Entry DOI: 10.2210/pdb3rsm/pdb
• Related: 1K2y 1K35 1P5D 1P5G
• Descriptor: Phosphomannomutase/phosphoglucomutase, ZINC ION, PHOSPHATE ION, ... (4 entities in total)
• Functional Keywords: isomerase, phosphohexomutase
• Biological source: Pseudomonas aeruginosa
• Total number of polymer chains: 1
• Total formula weight: 50554.85

Authors

Akella, A.,Anbanandam, A.,Kelm, A.,Wei, Y.,Mehra-Chaudhary, R.,Beamer, L.,Van Doren, S. (deposition date: 2011-05-02, release date: 2012-02-29, Last modification date: 2023-09-13)

Primary citation

Sarma, A.V.,Anbanandam, A.,Kelm, A.,Mehra-Chaudhary, R.,Wei, Y.,Qin, P.,Lee, Y.,Berjanskii, M.V.,Mick, J.A.,Beamer, L.J.,Van Doren, S.R.
Solution NMR of a 463-residue phosphohexomutase: domain 4 mobility, substates, and phosphoryl transfer defect.
Biochemistry, 51:807-819, 2012

PubMed Abstract: Phosphomannomutase/phosphoglucomutase contributes to the infectivity of Pseudomonas aeruginosa, retains and reorients its intermediate by 180°, and rotates domain 4 to close the deep catalytic cleft. Nuclear magnetic resonance (NMR) spectra of the backbone of wild-type and S108C-inactivated enzymes were assigned to at least 90%. (13)C secondary chemical shifts report excellent agreement of solution and crystallographic structure over the 14 α-helices, C-capping motifs, and 20 of the 22 β-strands. Major and minor NMR peaks implicate substates affecting 28% of assigned residues. These can be attributed to the phosphorylation state and possibly to conformational interconversions. The S108C substitution of the phosphoryl donor and acceptor slowed transformation of the glucose 1-phosphate substrate by impairing k(cat). Addition of the glucose 1,6-bisphosphate intermediate accelerated this reaction by 2-3 orders of magnitude, somewhat bypassing the defect and apparently relieving substrate inhibition. The S108C mutation perturbs the NMR spectra and electron density map around the catalytic cleft while preserving the secondary structure in solution. Diminished peak heights and faster (15)N relaxation suggest line broadening and millisecond fluctuations within four loops that can contact phosphosugars. (15)N NMR relaxation and peak heights suggest that domain 4 reorients slightly faster in solution than domains 1-3, and with a different principal axis of diffusion. This adds to the crystallographic evidence of domain 4 rotations in the enzyme, which were previously suggested to couple to reorientation of the intermediate, substrate binding, and product release.

PubMed: 22242625
DOI: 10.1021/bi201609n

Experimental method

X-RAY DIFFRACTION (2.1 Å)