3RLG

Crystal structure of Loxosceles intermedia phospholipase D isoform 1 H12A mutant

3RLG の概要

• エントリーDOI: 10.2210/pdb3rlg/pdb
• 関連するPDBエントリー: 3RLH
• 分子名称: Sphingomyelin phosphodiesterase D LiSicTox-alphaIA1a, MAGNESIUM ION, 1,2-ETHANEDIOL, ... (6 entities in total)
• 機能のキーワード: tim beta/alpha-barrel, plc-like phosphodiesterase, inactive mutant h12a phospholipase d, hydrolase
• 由来する生物種: Loxosceles intermedia (Spider)
• 細胞内の位置: Secreted: P0CE80
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34457.79

構造登録者

Giuseppe, P.O.,Ullah, A.,Veiga, S.S.,Murakami, M.T.,Arni, R.K. (登録日: 2011-04-19, 公開日: 2011-08-24, 最終更新日: 2024-11-20)

主引用文献

Ullah, A.,de Giuseppe, P.O.,Murakami, M.T.,Trevisan-Silva, D.,Wille, A.C.,Chaves-Moreira, D.,Gremski, L.H.,da Silveira, R.B.,Sennf-Ribeiro, A.,Chaim, O.M.,Veiga, S.S.,Arni, R.K.
Crystallization and preliminary X-ray diffraction analysis of a class II phospholipase D from Loxosceles intermedia venom.
Acta Crystallogr.,Sect.F, 67:234-236, 2011

PubMed Abstract: Phospholipases D are the major dermonecrotic component of Loxosceles venom and catalyze the hydrolysis of phospholipids, resulting in the formation of lipid mediators such as ceramide-1-phosphate and lysophosphatidic acid which can induce pathological and biological responses. Phospholipases D can be classified into two classes depending on their catalytic efficiency and the presence of an additional disulfide bridge. In this work, both wild-type and H12A-mutant forms of the class II phospholipase D from L. intermedia venom were crystallized. Wild-type and H12A-mutant crystals were grown under very similar conditions using PEG 200 as a precipitant and belonged to space group P12(1)1, with unit-cell parameters a = 50.1, b = 49.5, c = 56.5 Å, β = 105.9°. Wild-type and H12A-mutant crystals diffracted to maximum resolutions of 1.95 and 1.60 Å, respectively.

PubMed: 21301094
DOI: 10.1107/S1744309110050931

実験手法

X-RAY DIFFRACTION (1.6 Å)