3RLG
Crystal structure of Loxosceles intermedia phospholipase D isoform 1 H12A mutant
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Sphingomyelin phosphodiesterase D LiSicTox-alphaIA1a | polymer | 302 | 33672.6 | 1 | UniProt (P0CE80) Pfam (PF13653) In PDB | Loxosceles intermedia (Spider) | Dermonecrotic protein 1, LiRecDT1, Sphingomyelin phosphodiesterase D 1, SMD 1, SMase D 1, Sphingomyelinase D 1 |
2 | A | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
3 | A | 1,2-ETHANEDIOL | non-polymer | 62.1 | 4 | Chemie (EDO) | |||
4 | A | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 2 | Chemie (PEG) | |||
5 | A | TRIETHYLENE GLYCOL | non-polymer | 150.2 | 2 | Chemie (PGE) | |||
6 | water | water | 18.0 | 238 | Chemie (HOH) |
Sequence modifications
A: 2 - 285 (UniProt: P0CE80)
PDB | External Database | Details |
---|---|---|
Met -21 | - | expression tag |
Gly -20 | - | expression tag |
Ser -19 | - | expression tag |
Ser -18 | - | expression tag |
His -17 | - | expression tag |
His -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
Ser -11 | - | expression tag |
Ser -10 | - | expression tag |
Gly -9 | - | expression tag |
Leu -8 | - | expression tag |
Val -7 | - | expression tag |
Pro -6 | - | expression tag |
Arg -5 | - | expression tag |
Gly -4 | - | expression tag |
Ser -3 | - | expression tag |
His -2 | - | expression tag |
Met -1 | - | expression tag |
Leu 0 | - | expression tag |
Glu 1 | - | expression tag |
Ala 12 | His 38 | engineered mutation |
Asp 277 | Glu 298 | cloning artifact |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 33672.6 | |
Non-Polymers* | Number of molecules | 9 |
Total formula weight | 785.2 | |
All* | Total formula weight | 34457.8 |