3RJ1

Architecture of the Mediator Head module

3RJ1 の概要

• エントリーDOI: 10.2210/pdb3rj1/pdb
• 関連するPDBエントリー: 2HZM 2HZS
• 分子名称: Mediator of RNA polymerase II transcription subunit 11, Mediator of RNA polymerase II transcription subunit 17, Mediator of RNA polymerase II transcription subunit 8, ... (8 entities in total)
• 機能のキーワード: mediator, rna polymeras ii, pol ii, rnap, med, head module, mediator head, regulator, helical bundle, transcriptional regulation, rna polymerase ii, tbp, nucleus, transcription
• 由来する生物種: Saccharomyces cerevisiae (brewer's yeast,lager beer yeast,yeast); 詳細
• 細胞内の位置: Nucleus : Q99278 P32569 P38304 P32570 P32585 P34162 P38782
• タンパク質・核酸の鎖数: 21
• 化学式量合計: 636188.70

構造登録者

Imasaki, T.,Calero, G.,Cai, G.,Tsai, K.L.,Yamada, K.,Cardelli, F.,Erdjument-Bromage, H.,Tempst, P.,Berger, I.,Kornberg, G.L.,Asturias, F.J.,Kornberg, R.D.,Takagi, Y. (登録日: 2011-04-14, 公開日: 2011-07-27, 最終更新日: 2024-11-06)

主引用文献

Imasaki, T.,Calero, G.,Cai, G.,Tsai, K.L.,Yamada, K.,Cardelli, F.,Erdjument-Bromage, H.,Tempst, P.,Berger, I.,Kornberg, G.L.,Asturias, F.J.,Kornberg, R.D.,Takagi, Y.
Architecture of the Mediator head module.
Nature, 475:240-243, 2011

PubMed Abstract: Mediator is a key regulator of eukaryotic transcription, connecting activators and repressors bound to regulatory DNA elements with RNA polymerase II (Pol II). In the yeast Saccharomyces cerevisiae, Mediator comprises 25 subunits with a total mass of more than one megadalton (refs 5, 6) and is organized into three modules, called head, middle/arm and tail. Our understanding of Mediator assembly and its role in regulating transcription has been impeded so far by limited structural information. Here we report the crystal structure of the essential Mediator head module (seven subunits, with a mass of 223 kilodaltons) at a resolution of 4.3 ångströms. Our structure reveals three distinct domains, with the integrity of the complex centred on a bundle of ten helices from five different head subunits. An intricate pattern of interactions within this helical bundle ensures the stable assembly of the head subunits and provides the binding sites for general transcription factors and Pol II. Our structural and functional data suggest that the head module juxtaposes transcription factor IIH and the carboxy-terminal domain of the largest subunit of Pol II, thereby facilitating phosphorylation of the carboxy-terminal domain of Pol II. Our results reveal architectural principles underlying the role of Mediator in the regulation of gene expression.

PubMed: 21725323
DOI: 10.1038/nature10162

実験手法

X-RAY DIFFRACTION (4.3 Å)