3RBU

N-terminally AviTEV-tagged Human Glutamate Carboxypeptidase II in complex with 2-PMPA

Summary for 3RBU

Entry DOI	10.2210/pdb3rbu/pdb
Descriptor	Glutamate carboxypeptidase 2, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (10 entities in total)
Functional Keywords	peptidase, biotinylation, extracellular, hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor
Biological source	Homo sapiens (human)
Cellular location	Cell membrane; Single-pass type II membrane protein. Isoform PSMA': Cytoplasm: Q04609
Total number of polymer chains	1
Total formula weight	86480.67
Authors	Tykvart, J.,Sacha, P.,Barinka, C.,Starkova, J.,Knedlik, T.,Lubkowski, J.,Konvalinka, J. (deposition date: 2011-03-30, release date: 2012-01-04, Last modification date: 2023-09-13)
Primary citation	Tykvart, J.,Sacha, P.,Barinka, C.,Knedlik, T.,Starkova, J.,Lubkowski, J.,Konvalinka, J. Efficient and versatile one-step affinity purification of in vivo biotinylated proteins: Expression, characterization and structure analysis of recombinant human glutamate carboxypeptidase II. Protein Expr.Purif., 82:106-115, 2012 Cited by PubMed: 22178733 DOI: 10.1016/j.pep.2011.11.016 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.6 Å)

Structure validation