3R6N

Crystal structure of a rigid four spectrin repeat fragment of the human desmoplakin plakin domain

Summary for 3R6N

• Entry DOI: 10.2210/pdb3r6n/pdb
• Descriptor: Desmoplakin, (4S,5S)-1,2-DITHIANE-4,5-DIOL, 2,3-DIHYDROXY-1,4-DITHIOBUTANE (3 entities in total)
• Functional Keywords: spectrin repeat, sh3 domain, cell adhesion, desmosome
• Biological source: Homo sapiens (human)
• Cellular location: Cell junction, desmosome: P15924
• Total number of polymer chains: 2
• Total formula weight: 107196.45

Authors

Choi, H.-J.,Weis, W.I. (deposition date: 2011-03-22, release date: 2011-05-11, Last modification date: 2024-02-21)

Primary citation

Choi, H.J.,Weis, W.I.
Crystal structure of a rigid four-spectrin-repeat fragment of the human desmoplakin plakin domain.
J.Mol.Biol., 409:800-812, 2011

PubMed Abstract: The plakin protein family serves to connect cell-cell and cell-matrix adhesion molecules to the intermediate filament cytoskeleton. Desmoplakin (DP) is an integral part of desmosomes, where it links desmosomal cadherins to the intermediate filaments. The 1056-amino-acid N-terminal region of DP contains a plakin domain common to members of the plakin family. Plakin domains contain multiple copies of spectrin repeats (SRs). We determined the crystal structure of a fragment of DP, residues 175-630, consisting of four SRs and an inserted SH3 domain. The four repeats form an elongated, rigid structure. The SH3 domain is present in a loop between two helices of an SR and interacts extensively with the preceding SR in a manner that appears to limit inter-repeat flexibility. The intimate intramolecular association of the SH3 domain with the preceding SR is also observed in plectin, another plakin protein, but not in α-spectrin, suggesting that the SH3 domain of plakins contributes to the stability and rigidity of this subfamily of SR-containing proteins.

PubMed: 21536047
DOI: 10.1016/j.jmb.2011.04.046

Experimental method

X-RAY DIFFRACTION (2.95 Å)