3R6N
Crystal structure of a rigid four spectrin repeat fragment of the human desmoplakin plakin domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-11 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.9792 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 157.520, 82.520, 139.980 |
Unit cell angles | 90.00, 125.54, 90.00 |
Refinement procedure
Resolution | 45.653 - 2.950 |
R-factor | 0.2212 |
Rwork | 0.216 |
R-free | 0.26480 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.590 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.19) |
Phasing software | PHASER |
Refinement software | PHENIX (1.7_650) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.691 | 56.980 | 3.030 |
High resolution limit [Å] | 2.946 | 13.190 | 2.950 |
Rmerge | 0.046 | 0.033 | 0.365 |
Total number of observations | 910 | 5748 | |
Number of reflections | 30845 | ||
<I/σ(I)> | 16.3 | 18.3 | 2 |
Completeness [%] | 99.5 | 88.2 | 96.7 |
Redundancy | 2.9 | 2.8 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 16% PEG 8000, 0.1M Tris-Cl (pH 8.5), 4mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 293K |