3R2V

Crystal structure of polymerase basic protein 2 E538-R753 from Influenza A virus A/Yokohama/2017/03 H3N2

3R2V の概要

• エントリーDOI: 10.2210/pdb3r2v/pdb
• 関連するPDBエントリー: 2VY6 3KC6 3KHW 3L56
• 分子名称: PB2 C-terminal subunit, IODIDE ION, BORIC ACID, ... (4 entities in total)
• 機能のキーワード: structural genomics, seattle structural genomics center for infectious disease, ssgcid, influenza, flu, polymerase basic, pb2, h3n2, h1n1, h5n1, virus, viral protein, 2003 avian flu outbreak
• 由来する生物種: Influenza A virus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 25700.63

構造登録者

Seattle Structural Genomics Center for Infectious Disease (SSGCID) (登録日: 2011-03-14, 公開日: 2011-03-30, 最終更新日: 2023-09-13)

主引用文献

Smith, E.R.,Begley, D.W.,Anderson, V.,Raymond, A.C.,Haffner, T.E.,Robinson, J.I.,Edwards, T.E.,Duncan, N.,Gerdts, C.J.,Mixon, M.B.,Nollert, P.,Staker, B.L.,Stewart, L.J.
The Protein Maker: an automated system for high-throughput parallel purification.
Acta Crystallogr.,Sect.F, 67:1015-1021, 2011

PubMed Abstract: The Protein Maker is an automated purification system developed by Emerald BioSystems for high-throughput parallel purification of proteins and antibodies. This instrument allows multiple load, wash and elution buffers to be used in parallel along independent lines for up to 24 individual samples. To demonstrate its utility, its use in the purification of five recombinant PB2 C-terminal domains from various subtypes of the influenza A virus is described. Three of these constructs crystallized and one diffracted X-rays to sufficient resolution for structure determination and deposition in the Protein Data Bank. Methods for screening lysis buffers for a cytochrome P450 from a pathogenic fungus prior to upscaling expression and purification are also described. The Protein Maker has become a valuable asset within the Seattle Structural Genomics Center for Infectious Disease (SSGCID) and hence is a potentially valuable tool for a variety of high-throughput protein-purification applications.

PubMed: 21904043
DOI: 10.1107/S1744309111028776

実験手法

X-RAY DIFFRACTION (1.3 Å)